The diversity of blaZ gene as measured by the Simpson index of diversity (SID) was higher for the MRSA collection than for MSSA, although CDK inhibition not Entospletinib cell line statistically significant

due to the partial overlapping of the confidence intervals (SID = 79.18, 95%CI 69.6-88.8 vs SID = 76.09, 95%CI 61.3-90.9, respectively) – see Table 4. As illustrated by the allelic frequency distribution

per MRSA lineage (Figure 1) or the cluster tree of the thirteen blaZ alleles found in our collections (Figure 2), there is no clustering according to genetic lineages, as defined by MLST sequence type and SCCmec type, or MSSA/MRSA phenotype; i.e. the same allele could be detected in different genetic lineages or among MRSA and MSSA, and the same lineage could be characterized by several alleles. In addition, there was also no clear clustering of blaZ allotypes according to geographic origin or isolation date of the MRSA R406 research buy isolates (see Table 1). Table 3 Characteristics of bla locus alleles Gene Allele No. Frequency SNPc) Amino acid substitutions     MRSA a) MSSA b)   Silent Conservative Missense Nonsense   1 0.43 0.21 0 0 0 0 0   2 0.02 0 1 0 0 1 1   3 0.07 0.04 9 4 2 2 0   4 0.04 0 9 4 2 3 0   5 0.06 0 7 2 2 3 0   6 0.11 0.46 13 8 2 3 0 blaZ 7 0.02 0 12 6 2 4 0   8 0.10 0.04 11 6 2 3 0   9 0.07 0.08 20 9 2 7 0   10 Cyclooxygenase (COX) 0.04 0.04 19 8 2 7 0   11 0.06 0.04 24 11 3 8 0   12 0 0.04 24 11 2 8 0   13 0 0.04 12 7 2 3 0   1 0.33 0.45 0 0 0 0 0   2 0.15 0.25 6 5 0 1 0   3 0.19 0.15 1 0 0 1 0   4 0.19 0.05

4 3 0 1 0 blaI 5 0.04 0 7 5 0 2 0   6 0.07 0 4 3 0 1 0   7 0.04 0 5 4 0 1 0   8 0 0.05 3 1 1 1 0   9 0 0.05 1 0 0 1 0   1 0.26 0.24 0 0 0 0 0   2 0.07 0 19 9 4 6 0   3 0.10 0 18 7 4 6 0   4 0.07 0.06 35 15 9 10 0   5 0.07 0.18 35 15 7 11 0   6 0.07 0.12 17 6 4 6 0 blaR1 7 0.07 0.06 24 10 7 7 0   8 0.03 0 33 12 6 12 0   9 0.16 0 31 11 6 11 0   10 0.13 0.24 32 12 6 11 0   11 0 0.06 20 9 5 7 0   12 0 0.06 34 16 6 10 0 a) The total number of MRSA strains whose blaZ, blaI and blaR1 genes were analyzed is 54, 27 and 31, respectively. b) The total number of MSSA strains whose blaZ, blaI and blaR1 genes were analyzed is 24, 20 and 17, respectively.