In few occasions it was not possible to group those strains into a family with certainty, therefore SNP detection in Ag85C103 and mgtC182 was needed. Thus, regarding SCG-3b, the most prevalent in our community, the addition of a specific SNP detection as mgtC182, a characteristic SNP of the Haarlem family, gave more specific information. Filliol and collaborators joined in this SCG-3b basically Haarlem isolates, but also some T, LAM, and orphan strains . It either happened the same learn more concerning SCG-5, the second most prevalent
SCG in Aragon, in which Filliol and collaborators included essentially LAM strains, but also T, Haarlem, S, unknown and orphan isolates . The pyrosequencing method applied allows to include an isolate in SCG-5, further the Ag85C 103 asserts of its LAM membership even if spoligotyping had not been detected it at first. Selleck 4SC-202 Regarding SCG-6a, which was the third group of relevance in our study, we believe it includes the
vast majority of the T isolates that would group as the “authentic T” isolates, being a more evolved strains since they belong to the PGG-3. Another achievement of this SNPs set has been the discovery of the two genetically and epidemiologically not linked isolates included in the new “SCG-6c”. It suggests that the tubercle bacillus is incessantly varying and highlights the
value of SNPs to follow the evolution of M. tuberculosis complex. Concerning the PGG determination, around 70% of the strains circulating in our community grouped in the PGG-2. oxyclozanide This study provides a first inside into the AICAR nmr structure of the M. tuberculosis population in Aragon and Spain. The strains causing the largest clusters were classified as belonged to PGG-3, ARA7 (SCG-6a) and ARA21 (SCG-6c), what means these modern strains are causing the more cases of TB in our region, both of them belong to the Euro-American lineage [19, 25]. Comparing our results with a study carried out in London , we appreciate less diversity regarding Spoligo-families probably due to the minor rate of patients that born abroad in respect to the London population. They characterised the MTBC strains using SNPs, however some of the isolates remained unclassified. A recent publication designed an algorithmic differentiating Euro-American based on polymorphic SNPs in 5 genes in an extend collection of well-classified members of the MTB complex . However, the application of the analysis of the set of SNPs previously described [8, 17, 21] selected in this study allowed us to assign 75 strains sharing different spoligotypes to different SCGs and families in the MTC, specially those assigned to the ill defined T and other unclassified.