with available complete genome sequences, stability within this bacterium and prevalence across X. arboricola pv. pruni genotypes, but absence in any other pathovar provides a potential target for pathovar-level detection CT99021 mouse and identification of this
regulated quarantine pathogen. The authors thank P. Llop for helpful discussions. J.B. was supported by the German Federal Ministry of education and Research (grant 0315599B ‘Genomik-Transfer’). This study was financed by the Swiss Secretariat for Education and Research (SBF COST C07.0139) and was conducted within the European Science Foundation research network COST Action 873. Table S1. Orthologs of type III effectors or helper proteins found in plasmid pXap41 with GenBank locus tags of orthologous genes found in other Xanthomonas genomes. Please note: Wiley-Blackwell is not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article. “
“Among the tailed phages, the myoviruses, those with contractile tails, are widespread and diverse. An important component of the Myoviridae family is the genus ‘T4-like viruses’. The present study
was aimed at elucidating the molecular diversity of T4-type bacteriophages in Lake Baikal by partial sequencing of g23 genes of T4-type bacteriophages. Our study revealed that the g23 gene sequences investigated were highly diverse and different from those of T4-like bacteriophages and from g23 clones obtained from different environments. C59 wnt research buy Phylogenetic analysis showed that all g23 fragments from
Lake Baikal, except for the one sequence, were more closely related to marine T4 cyanophages and to previously described subgroups of uncultured T4 phages from marine and rice field environments. Tailed bacteriophages are the most abundant biological entities in marine environments (Breitbart et al., 2002). Among the tailed phages, the myoviruses, those with contractile tails, are widespread and diverse. For example, the environmental sequences belonging to the Myoviridae family represent 11–23% of all sequences obtained from metagenomic analysis of uncultured Pacific viral samples (Breitbart et al., 2002). According to the virus taxonomy Ribociclib mw and nomenclature approved by the International Committee on Taxonomy of Viruses, the family of Myoviridae is composed of seven genera (http://www.ncbi.nlm.nih.gov/ICTVdb/Ictv/fs_index.htm). An important component of the Myoviridae family in particular from an ecological viewpoint is the genus ‘T4-like viruses’. T4-like phages are a diverse group of lytic bacterial viruses that share genetic homologies and morphological similarities to the well-studied coliphage T4 (Ackermann & Krisch, 1997). These phages have been divided into subgroups (T-evens, PseudoT-evens, SchizoT-evens and ExoT-evens) according to the sequences of their virion genes (Monod et al.