Our study revealed that the protein was internalized after 90 min of incubation, mostly in hyphal tips, but also within hyphal segments (Figure 6A, B). The protein seemed not to localize to
cell compartments, but was distributed in the cytoplasm. Similar results were obtained with A. niger wild type (data not shown). Control experiments proved the specificity of the intracellular immunofluorescent signals: no intracellular fluorescent signals were detected in samples where either AFPNN5353 (Figure 6C, D) or the primary antibody or the secondary antibody was omitted (data not shown). Figure 6 Indirect immunofluorescence staining of A. nidulans with rabbit anti-AFP NN5353 antibody. Fungi were incubated with 0.2 μg/ml AFPNN5353 (A, E, buy CA-4948 G) or without antifungal protein (C). 20 μg/ml latrunculin B (E) and 10 mM Ca2+ (G) significantly reduced protein uptake. (B, D, F, H) are the respective light AZD1390 purchase microscopic Tideglusib solubility dmso images of (A, C, E, G). Scale bar 10 μm. To analyse the AFPNN5353 localization in more detail, A. nidulans was incubated with AFPNN5353 in the presence of latrunculin B, a potent inhibitor of actin polymerization and endocytosis [[35–37]]. At low latrunculin B concentrations (5 μg/ml), protein uptake was severely reduced compared to the positive control without latrunculin
B (data not shown), whereas 20 μg latrunculin B/ml completely inhibited the uptake of 0.2 μg/ml AFPNN5353. The solvent of latrunculin B, DMSO, had no adverse effect on protein uptake (data not shown). This indicates that AFPNN5353 enters the A. nidulans cells by an endocytotic mechanism (Figure 6E, F). Based on our observation that Ca2+ ions antagonize the growth inhibitory activity of AFPNN5353, we questioned whether Ca2+ prevents actin-mediated internalisation
of the antifungal protein. Indeed, the presence of 10 mM CaCl2 inhibited protein uptake (Figure 6G, H). Most interestingly, no specific fluorescent signals were detectable in M. circinelloides when treated with up to 500 μg/ml of antifungal protein (data not shown), indicating that AFPNN5353 does not bind aminophylline to insensitive strains. Discussion In this study we provide important insights into the mechanistic basis of AFPNN5353, a AFP homologous protein. Species specificity tests revealed that AFPNN5353 is active against a broad range of filamentous fungi, including human and plant pathogens. Although the proteins AFPNN5353 and AFP are almost identical and show a similar toxicity, MICs for AFPNN5353 differed slightly from those reported for AFP . We attribute this discrepancy to differences in the experimental setups, e.g. fungal strains, medium composition, conidial inoculum, incubation times, cultivation temperature etc., rather than to the differences in the primary sequence of both proteins.