41%) were H. pylori positive. The prevalence reached a peak at the age of 30–39 years (90.82%). There was significant difference between sexes and women had a higher infection rate than men. The prevalence of

H. pylori infection was also associated with eating kipper food and fried food. No association between H. pylori prevalence and smoking or drinking was found. Compared to healthy individuals, people with dyspeptic diseases (peptic ulcer, gastroenteritis) click here presented a high prevalence of H. pylori infection. Using multivariate logistic regression analysis, age, history of peptic ulcer and gastroenteritis were the independent predictors for H. pylori infection. Conclusion: Yangzhong country, a persistent high risk area of gastric carcinoma, had a high prevalence of H. pylori infection and was related to several risk factors. The underlying mechanisms are needed to be further investigated. Key Word(s): 1. Helicobacter pylori; 2. risk factor; 3. Jiangsu province; 4. gastric cancer; Presenting Author: YIN ZHU Additional Authors: HONGSHENG WANG, YAN SUN, MING YAN, JUNGSOO JOO, JIAFANG SUN, WEIPING CHEN, CHENFENG QI, NONGHUA LV, HERBERTCARPENTER Paclitaxel manufacturer MORSE, III, WILLIAMG COLEMAN, JR Corresponding Author: HERBERTCARPENTER MORSE, III,, WILLIAMG COLEMAN, JR Affiliations: National Institutes of Health, NIDDK; the First Aiilated Hospital of NanChang Univerisity, the Department of

Gastroenterology; National Institutes of Health, NIAID; the First Affiliated Hosptial of Nanchang University,

the Department of Gastroenterology Objective: Interferon Regulatory Factor 8 (IRF8) is a transcription factor Avelestat (AZD9668) of the interferon regulatory factor (IRF) family and has many functions involved in the regulation of development, growth and host defense in hematopoietic lineage cells including innate and adaptive immune responses. However, expression and function of IRF8 in non-hematopoietic cells remain poorly understood. Methods: We used microarray analysis to monitor host responses to Helicobacter pylori (H. pylori) infection. The expression of IRF8 was detected by quantitative PCR and western blot in the gastric epithelial cell line (GSM06) and macrophage cell line (RAW264.7) infected by H. pylori. We generated an IRF8-EGFP fusion protein reporter mice (IRF8-EGFP mice) to monitor IRF8 expression during H. pylori infection using immunofluorescence and qPCR. Results: The results of microarray analysis showed that the IRF8 gene was up-regulated (fold changes > 2, p < 0.0001). Both IRF8 mRNA expression and IRF8 protein level increased in GSM06 and RAW264.7 after infected by H. pylori. The protein of IRF8 expressed in gastric epithelial cells from IRF8-EGFP mouse stomach, mostly localized in the nucleus. The fluorescence of IRF8 increased on gastric epithelial cells with the extension of H. pylori infection. IRF8 mRNA expression of stomach tissues increased significantly in IRF8-EGFP mice infected by H. pylori, compared with wild type control mice (p < 0.05).