Herein, we report, for the first time, the discovery of β-D-2′-C-methyl-4-N-hydroxycytidine
phosphoramidate nucleotides as non-toxic anti-HCV agents that upon intracellular metabolism deliver multiple nucleoside inhibitor 5′-triphosphates (NI-TP). Anti-infection Compound Library price Methods: A variety of β-D-2′-C-methyl-4-N-OH-C prodrugs were synthesized and evaluated for: 1) inhibition of HCV viral replication in Huh7 replicon cells; 2) cytotoxicity in various cell lines; 3) cellular pharmacology in both Huh7 cells and primary human hepatocytes; and 4) IC50 values for three NI-TPs were determined against the HCV NS5B polymerase (Pol). Results: The β-D-2′-C-methyl-4-N-OH-C prodrugs were pan-genotypic, effective against various HCV resistant mutants and resistant variants could not be selected in a Huh7 based replicon system. Upon cellular entry, β-D-2′-C-methyl-4-N-OH-C prodrugs were metabolized to generate three distinct NI-TPs: 2′-C-methyl-CTP, 2′-C-methyl-UTP and 2′-C-methyl-4-N-OH-CTP. The two former NI-TPs are well characterized for their anti-HCV activity, but interestingly, we found that 2′-C-methyl-4-N-OH-CTP
can be incorporated both as a cytidine and uridine analog with HCV pol. We also established that 2′-C-methyl-4-N-OH- CTP was able to effectively inhibit RNA polymerization when pre-incubated with purified HCV pol, but was outcompeted when co-incubated with natural CTP and UTP substrates. Conclusion: The discovery of these novel β-D-2′-C-methyl-4-N-OH-C prodrugs could have learn more important clinical implications based on their unique ability to deliver a cascade of three active pan-ge-notypic NI-TPs intracellularly with distinctive incorporation profiles and high barrier to resistance. Disclosures: Raymond F. Schinazi – Board Membership: RFS Pharma, LLC; Stock Shareholder: RFS Pharma, LLC Tony Whitaker – Employment: RFS Pharma, LLC Tami R. McBrayer – Employment: RFS Pharma Steven J. Coats – Employment: RFS Pharma The following people have nothing to disclose: Franck Amblard, Sijia Tao, Maryam Ehteshami, Sheida Amiralaei, Hao Li, Jadd Shelton Background A once
daily fixed-dose combination tablet (FDC) of NS5A inhibitor ledipasvir (LDV) 90 mg and NS5B inhibitor sofosbuvir Bacterial neuraminidase (SOF) 400 mg is being developed for the treatment of chronic HCV infection. Methods The drug-drug interaction (DDI) profile of FDC has been characterized using in vitro data, Phase 1 DDI results in healthy subjects and Phase 2/3 population pharmacokinetic (popPK) analyses in HCV-in-fected patients. The Phase 1 program evaluated DDIs between FDC or components, and HIV antiretrovirals (ARVs), oral contraceptives (OCs), acid-reducing agents, immunosuppressants (IST), opiates and rifampin (RIF). The effect of anticoagulants, selective serotonin reuptake inhibitors (SSRIs), calcium channel blockers (CCB), statins and diuretics on FDC PK was assessed by popPK analyses.