Cryobacterium, Rhodococcus, and Veillonella were identified only in the ovary, whereas Anaerobiospirillum was the only genera unique to the gut. The molecular approach applied in this study allowed us to assess the relative abundance of the microbiota associated with R. microplus. The KU-57788 clinical trial predominant genera in the bacterial communities of the

tick samples analyzed based on an abundance cutoff of 1.0% are shown for each sample in Figure 2. Staphylococcus was relatively abundant (> 18%) in adult males and eggs, but not in adult female ticks. Other prevalent genera were Corynebacterium (> 13%) in eggs and adult males, and EGFR inhibitor Coxiella (> 13%) in tick eggs. Achromobacter (27.7%), Pseudomonas (12.6%), and Sinorhizobium (7.7%) were the predominant genera found in adult female ticks. Among the tissues sampled, Coxiella was the most abundant (98.2%) genus in ovary, whereas Anaerobiospirillum (29.5%) and Brachybacterium (21.9%) predominated in the tick gut. Other

relatively less abundant genera, but worth noting, include Borrelia (7.9%) in the tick gut; Clostridium (3.9%) in adult female ticks; Escherichia (1.5%) in the tick gut; Klebsiella (1.3%) in adult female ticks; Streptococcus in eggs (2.9%) and adult males (1.%); Enterococcus in adult male ticks (1.4%), adult female ticks (2.2%), and tick gut (11.4%); and Wolbachia in adult female ticks (1.8%). Figure 2 Relative abundance of bacterial genera in life stages and tissue samples from R. microplus as detected by bTEFAP pyrosequencing. a) Adult female cattle tick. Mean percentages (n = 2). Values below 1% were grouped as “”Other”" with total value of 9.5%. “”Other”" group includes: Staphylococcus (0.7%), check details Bacillus (0.5%),

Streptococcus (0.7%), Vagococcus (0.3%), Pseudobutyrivibrio (0.7%), Nocardioides (0.2%), Asteroleplasma (0.9%), Ruminococcus (0.4%), Escherichia (0.9%), Acetivibrio (0.3%), Erwinia (0.1%), Pedobacter (0.2%), Dermabacter (0.1%), Ornithinicoccus (0.2%), Oribacterium (0.7%), Alkaliflexus (0.2%), Paludibacter (0.5%), Pantoea (0.2%), Cytophaga (0.1%), Mitsuokella (0.1%), PLEK2 Enterobacter (0.1%), Paucisalibacillus (0.4%), Lachnobacterium (0.1%), Caldithrix (0.2%), Shigella (0.1%), Solirubrobacter (0.1%), Rhodobacter (0.1%), Desulfosporosinus (0.1%). b) Adult male cattle tick. Mean percentages (n = 2). Values below 1% were grouped as “”Other”" with total value of 3.8%. “”Other”" group includes: Coxiella (0.1%), Prevotella (0.3%), Rikenella (0.1%), Pseudomonas (0.2%), Escherichia (0.3%), Hallella (0.3%), Pantoea (0.1%), Moraxella (0.7%), Arthrobacter (0.1%), Enhydrobacter (0.1%), Mogibacterium (0.1%), Kocuria (0.5%), Enterobacter (0.1%), Exiguobacterium (0.2%), Lysinibacillus (0.1%), Belnapia (0.1%). c) Cattle tick egg. Mean percentages (n = 3). Values below 1% were grouped as “”Other”" with total value of 6.9%. “”Other”" group includes: Achromobacter (0.3%), Enterococcus (0.1%), Clostridium (0.1%), Serratia (0.7%), Ruminococcus (0.3%), Propionibacterium (0.4%), Klebsiella (0.2%), Acetivibrio (0.