0–66.7%) and 29.9% (range across study period 0.0–53.1%) from Western part of India. The difference reported from the four regions is statistically significant having two-tailed P value of 0.0342 using the Chi-square test. No statistically significant differences were observed between regions by gender. Of the 4711 cases of acute severe gastroenteritis recorded, all study sites combined

reported the highest number of cases in the month of May 2012 and the lowest number of cases in the month of April 2011 (Fig. 3). Northern, southern, eastern and western parts of India reported highest numbers of cases in the months of June 2012, July 2012, May 2012 and June 2012 respectively while they reported lowest number of cases in the months of March 2012, August 2011, April

2011 and November 2011, respectively. A distinct KU-57788 chemical structure seasonality of rotavirus positivity was observed in different parts of India with peak months of rotavirus hospitalization from December through February in north, east and western parts of India. In south India, rotavirus hospitalizations were observed throughout the year without any distinct seasonal peak (Fig. 2). Rotavirus related hospitalizations were highest from October through March for all the regions (Table 3). Strain characterization PI3K inhibitor by ELISA for all stool samples that tested positive for rotavirus VP6 antigen was carried out. Genotyping was performed at the Central Laboratory using reverse-transcription Rolziracetam polymerase chain reaction (RT-PCR). The most dominant genotype was G1P8 (23.84%) followed by G2P4 (12.93%) and G9P4 (8.13%) (Fig. 4 and Table 4). The age specific analysis of genotyping revealed differences with increasing age: rotavirus infections due to G12P6, which were responsible for 7% of cases across all age groups, contributed toward 21% of burden in children less than 6 months. This decreased to 8% in the age group 6–11 months and around 2–3% in children older than 12 months of age. Across all age groups, mixed infections were responsible for nearly 25%

of the positive cases (Fig. 5). This study used a standardized approach based on the generic protocol for hospital-based surveillance to estimate the burden of rotavirus gastroenteritis in children [4]. On an average rotavirus antigen was detected in 26.4% (ranging from as high as 52.5% to as low as 10.3%) of all diarrhea-related hospital admissions among children aged less than 5 years during 16 months study period. Overall 80% of rotavirus positive cases occurred among children less than 2 years old. Taking into account one complete calendar year from August 2011 to July 2012, rotavirus antigen was detected in 27.6% (ranging from as high as 52.5% to as low as 15.3%) of all diarrhea related hospital admission among children aged less than 5 years. A review of studies performed in India during 1990–2005 estimated that rotavirus disease accounted for 20.8% of all diarrhea related hospital admissions [5] whereas Kang et al.