dentium BMN 673 ic50 Dental caries BS 16 B. dentium Adult feces BS 39 B. dentium Adult feces BS 72 B. dentium Adult feces Crohn 24 B. dentium Adult feces NCTC 11818T B. longum Adult feces BS 101 B. longum Adult feces DSMZ 20438T B. pseudocatenulatum Infant feces B2b B. pseudocatenulatum Adult feces C19i B. pseudocatenulatum Child feces C20b B. pseudocatenulatum Child feces C1c SN-38 supplier B. pseudocatenulatum Child feces *: Received from B. Biavati, Instituto di Microbiologia Agaria e Tecnica, Università degli Studi di Bologna, Bologna, Italy ATCC : American Type Culture Collection, Rockville, Maryland, USA ; CCUG : Culture Collection, University

of Göteborg, Göteborg, Sweden; DSMZ : Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Göttingen, Germany ; NCTC : National Collection of Type Cultures, Central Public Health Laboratory, London; England); NCFB : National Collection of Food Bacteria, Shinfield, Reading, Berks, England The PCR RFLP patterns based on 16S rDNA were validated in a previous study [20]. The RFLP patterns observed (i) with AluI were named II (600-200-150-100 bp) and V (5-95-152-206-285-311), (ii) with TaqI were

VIII (470-330-250 bp), IX (470-250-210-120 bp) and X (132-200-664). The II-VIII pattern was attributed to B. pseudolongum and the II-IX pattern to bifidobacteria from human origin. Detection of total bifidobacteria – St-Marcellin process (Vercors’s plant) Out of the 176 analyzed samples, EPZ015938 in vivo 153 (87%) were positive with PCR based on 16S rDNA and 154 (88%) were positive with PCR on the hsp60 gene (Table 2). Percentages of positive samples were very similar using one or the other method and at each studied step, from 80% (step C, after removal from the mold) to 95%, in raw milk samples. (step A). Table 2 Number (percentage) of samples containing total bifidobacteria and B. pseudolongum in St-Marcellin and Brie processes Process/Methods   Production steps St-Marcellin Total n = 176 A Mirabegron n = 44 B n = 44 C n = 44 D n = 44 Total bifidobacteria           PCR 16S rDNA 153 (87%) 42 (95%) 37 (84%) 35 (80%)

39 (89%) PCR hsp60 gene 154 (88%) 42 (95%) 38 (86%) 35 (80%) 39 (89%) B. pseudolongum           PCR RFLP (16S rDNA) 135 (77%)/ 41 (93%)/ 28 (66%)/ 34 (77%)/ 32 (73%)/ Real time PCR (hsp60 gene) 120 (68%) 35 (80%) 27 (61%) 27 (61%) 31 (70%) Brie Total n = 120 A’ (n = 30) B’ (n = 30) C’ (n = 30) D’ (n = 30) Total bifidobacteria           PCR 16S rDNA 107 (89%) 29 (97%) 21 (70%) 28 (93%) 29 (97%) PCR hsp60 gene 105 (88%) 29 (97%) 22 (73%) 27 (90%) 27 (90%) B. pseudolongum           PCR RFLP (16S rDNA) 107 (89%) 29 (97%) 21 (70%) 28 (93%) 29 (97%) Real time PCR (hsp60 gene) ND ND ND ND ND St-Marcellin/Production steps: A, raw milk; B, after addition of rennet; C, after removal from the mold; D, ripening (Day 21) Brie/Production steps: A’, raw milk; B’, after second maturation; C’, after removal from the mold; D’, ripening (Day 28) NT, not done A significant decrease of bifidobacteria positive samples (F = 169; P ≤ 0.