coli (EHEC) O157:H7 Sakai strain isolated during an selleck inhibitor outbreak in Sakai City, Japan, in 1996 was studied [30]. coli belonging to different pathovars click here and phylogenetic groups during exponential growth in pooled human urine and LB broth Urine LB broth Urine vs LB Strains Phylo-groups Virulence groups TBARS* p** TBARS p p ABU 57 A ABU
9.0 ± 1.7 p = 0.835 7.1 ± 0.7 p = 1.000 p = 0.995 ABU83972 B2 ABU 7.3 ± 1.0 6.4 ± 0.1 p = 1.000 ABU 5 D ABU 7.2 ± 1.1 p = 1.000 6.3 ± 1.1 p = 1.000 p = 1.000 IH11128 B2 UPEC 6.7 ± 2.0 p = 1.000 6.1 ± 0.4 p = 1.000 p = 1.000 IAI 74 B2 UPEC 6.6 ± 0.9 p = 1.000 6.1 ± 0.7 p = 1.000 p = 1.000 ABU 63 B2 ABU 6.6 ± 1.2 p = 1.000 5.8 ± 0.9 p = 1.000 p = 1.000 IAI 39 D UPEC 6.6 ± 1.5 p = 1.000 7.1 ± 0.7 p = 1.000 p = 1.000 CFT073 B2 UPEC
6.3 ± 0.9 p = 0.999 5.9 ± 0.7 p = 1.000 p = 1.000 ABU 27 B2 ABU 5.6 ± 0.9 p = 0.905 4.9 ± 0.5 p = 0.976 p = 1.000 ABU 64 B2 ABU 5.6 ± 0.4 p = 0.866 6.8 ± 0.2 p = 1.000 p = 1.000 UMN 026 D UPEC 5.2 ± 1.1 p = 0.458 6.2 ± 0.6 p = 1.000 p = 1.000 ED1a B2 commensal 5.2 ± 1.1 p = 0.542 4.9 ± 0.2 p = 0.979 p = 1.000 536 B2 UPEC 5.1 ± 1.0 p = 0.458 Thiazovivin manufacturer 6.3 ± 1.7 p = 1.000 p = 1.000 J96 B2 UPEC 5.0 ± 1.1 p = 0.653 5.3 ± 1.4 p = 0.998 p = 1.000 ABU 20 B2 ABU 4.9 ± 0.9 p = 0.307 4.3 ± 1.2 p = 0.743 6-phosphogluconolactonase p = 1.000 IAI1 B1 commensal 4.3 ± 0.7 p = 0.075 4.4 ± 0.3 p = 0.789 p = 1.000 Sakai E EHEC 3.9 ± 0.4 p = 0.016 4.6 ± 0.7 p = 0.900 p = 1.000 UTI 89 B2 UPEC 3.8 ± 0.6 p = 0.015 5.1 ± 0.1 p = 0.996 p = 1.000 ABU 38 B1 ABU 3.8 ± 0.8 p = 0.012 4.5 ± 0.1 p = 0.838 p = 1.000 ABU 62 B1 ABU 3.5 ± 0.9 p = 0.005 6.1 ± 0.8
p = 1.000 p = 0.560 MG1655 A K-12 laboratory strain 2.6 ± 0.5 p = 0.0001 6.3 ± 1.4 p = 1.000 p = 0.546 * TBARS values are expressed in micromoles per 1011 cells. Midstream urine was collected over 24 hours from healthy male volunteers, with no history of UTI or antibiotic use in the last two months. The urine was pooled, centrifuged, filtered (filter size 0.22μm) and stored at −20°C. The growth experiments were assayed using 96 well plates and OD600 was measured with a Tecan Infinite M200 plate reader.