The sizes of the class 1 integron amplicons, which correspond to the approximate sizes of the cassette regions, were between 0.7 kb and 2 kb. Seven different Dibutyryl-cAMP mw cassettes were identified, LY2874455 including the dfr gene that encodes resistance to trimethoprim and the aadA gene

that encodes resistance to streptomycin. The two genes most frequently associated with each other were dfrA17 and aadA5 (11/25, 22.4%) (Table 2). Table 2 Characteristics of ESBL-producing Enterobacteriaceae isolates and their associated drug resistance genes and gene cassettes     ESBLs Other β-lactamases Associated drug resistance genes Gene cassettes Species No CTX-M-15 SHV-12 Both TEM-1 OXA-1 TetA aac6′-1b aac6′-1b-cr qnrA qnrB catB3 sul1 sul2 sul1- sul2

aadA1 aadA2 aadA4 aadA5 dfrA5 drA22 dfrA17-aadA5 E. coli 18 14 2 2 12 13 8 14 13 0 3 0 2 3 8 NVP-BGJ398 mouse 2 1 1 1 2 0 6 K. pneumoniae 14 6 3 5 7 13 9 13 13 0 5 4 2 5 7 0 2 0 0 1 1 3 K. oxytoca 3 1 2 0 1 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0 0 0 E. cloacae 14 8 4 1 12 2 7 8 7 1 4 0 0 6 8 0 1 1 0 0 0 2 Totals 49 29 11 8 32 28 24 35 33 1 12 4 4 14 25 2 4 2 2 3 1 11 Resistance transfer Transfer of ESBL by conjugation to E. coli J53-2 was successful for 29 (59.2%) of the 49 ESBL isolates, which consisted of eight E. coli, eight E. cloacae and 12 K. pneumoniae isolates and one K. oxytoca isolate. ESBL transfer by plasmid DNA electroporation into E. coli DH10B was Epothilone B (EPO906, Patupilone) successful for five (10.2%) of the 20 remaining isolates; four were E. coli isolates and one was a K. pneumoniae isolate. The presence of bla CTX-M, bla SHV, bla TEM and bla OXA was confirmed by PCR in the 34 transconjugants and transformants. Transfers of non-ESBL resistance genes (tetracycline, gentamicin and trimethoprim-sulfamethoxazole) were also

detected by antimicrobial susceptibility testing. Plasmid replicon type determination PCR-based replicon typing in the 34 transconjugants and transformants demonstrated the presence of the IncFII, HI2 and FIA replicons in these isolates (Table 3). IncFII was the most prevalent replicon type and was detected in 20 (58.8%) (10 E. coli and 10 K. pneumoniae) of the 34 isolates. HI2 was found in 13 (38.2%) isolates (eight E. cloacae, three K. pneumoniae, one E. coli and one K. oxytoca) and FIA was found in one E. coli isolate. The plasmids carrying bla CTX-M-15 were assigned to the FII (n=12) and HI2 (n=8) replicon types. Plasmids carrying bla SHV-12 (n=5) or carrying both bla CTX-M-15 and bla SHV-12 (n=2) were assigned to FII. Table 3 β-lactamase genes transferred to transconjugants and electroporants and their replicon type β-lactamase genes Replicon type Transconjugants   Electroporants   E. coli K. pneumoniae K. oxytoca E. cloacae Totals E. coli K.