Fluorescent microscope was employed
to observe the transfection results. At 48 hours after transfection, RNAs and proteins R428 cost were extracted; Then the expressions of XPD, DNp73 and GADD45β were detected by RT-PCR and Western blotting, respectively. The cell proliferation was assessed by MTT assay. The changes in cell apoptosis were evaluated by flow cytometry. Results: Green Fluorescent Protein (GFP) was expressed in SMMC-7721-pEGFP-N2-XPD group cells and SMMC-7721-pEGFP-N2 group cells. In contrast, GFP was undetectable in Lip group and Blank group cells by fluorescent microscope. Compared with Blank group, Lip group and N2 group, the expression of DNp73 mRNA decreased significantly in XPD group (P < 0.01), but the expressions of XPD and GADD45β mRNAs were enhanced obviously in XPD group (P < 0.01). There was no statistical diffirences of XPD, DNp73 and GADD45βmRNAs expressions among Blank group, Lip group and N2 group (P > 0.05). The trend of XPD, DNp73 and GADD45β proteins detected by Western blotting were consistent with the trend of their mRNAs. The proliferation of SMMC-7721 cells
was markedly inhibited (P < 0.01) and the apoptosis of SMMC-7721 cells was increased after transfected by XPD (P < 0.01). Conclusion: The wild-type XPD as an tumor suppressor gene plays an inhibitory role in the carcinogenesis of HCC. The expression Vincristine of DNp73 decreased and the expression of GADD45βincreased with the overexpression of XPD, suggests that both of them may play a key role in the mechanism of XPD inhibiting the carcinogenesis of HCC. Key Word(s): 1. Hepatoma; 2. XPD gene; 3. DNp73 gene; 4. GADD45b gene;
Presenting Author: ANILK JOHN Additional Authors: MADIHAEMRAN SOOFI, SAADAL KAABI, ESRAMOHD AL ADHAL, SALWA KANDATH, MOUTAZ DERBALA, MT BUTT, RAFIE YAKOOB, Flavopiridol (Alvocidib) MUNEERA MOHANNADI, HAMID WANI Corresponding Author: ANILK JOHN Affiliations: Hamad Medical Corporation; Hamad Medical Corporation; Hamad Medical Corporation; Hamad Medical Corporation; Hamad Medical Corporation; Hamad Medical Corporation; Hamad Medical Corporation; Hamad Medical Corporation; Hamad Medical Corporation; Hamad Medical Corporation Objective: Percutaneous liver biopsy is the standard of care for obtaining hepatic tissue for histopathological assessment of parenchymal liver disease. Today percutaneous liver biopsies are mostly performed under real time ultra sound guidance by radiologists. We prospectively audited our practice of blind outpatient liver biopsies, performed without image guidance by gastroenterologists for its safety and adequacy, in this era of high tech imaging. Methods: All patients who underwent blind percutaneous liver biopsies without image guidance by gastroenterologists for grading and staging of chronic hepatitis B/C were audited prospectively for the safety of the procedure and the adequacy of tissue samples for proper grading and staging.